|
KMID : 0545120020120020340
|
|
Journal of Microbiology and Biotechnology
2002 Volume.12 No. 2 p.340 ~ p.344
|
|
|
Cloning and Expression of a Paenibacillus sp. Neopullulanase Gene in Saccharomyces cerevisiae Producing Schwanniomyces occidentalis Glucoamylase
|
|
Kim, Hyo Jeong
Park, Jeong Nam/Kim, Hee Ok/Shin, Dong Jun/Chin, Jong Eon/Lee, HwangHee Blaise/Chun, Soon Bai
|
|
|
Abstract
|
|
|
|
A gene, npl, encoding neopullulanase from Paenibacillus sp. KCTC 8848P was cloned and expressed in Escherichia coli. It consisted of an open reading frame of 1,530 bp for a protein that consisted of 510 amino acids with a molecular weight of 58,075 Da. The deduced amino acid sequence of the neopullulanase gene had 92% identity with the neopullulanase of Bacillus polymyxa. The npl gene was also expressed in Saccharomyces cerevisiae secreting Schwanniomyces occidentalis glucoamylase (GAM1) under the control of the yeast actin gene (ACT1) promoter. Secretion of the neopullulanase was directed by the yeast mating pheromone α-factor (MFα1) prepro region. Enzyme assays confirmed that co-expression of npl and GAM1 enhanced starch and pullulan degradation by S. cerevisiae.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|